ptAD-seq-ubi63E-Gal4-DBD
(Plasmid #111930)

• Purpose: Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.
• Depositing Lab: Alexander Stark
• Publication: Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.201798896. doi: 10.15252/embj.201798896. Epub 2018 Jul 13.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Total vector size (bp): 7238
• Modifications to backbone: We derived the tAD-seq Gal4-DBD-candidate expression plasmid from the fly STARR-seq plasmid (AddgeneID 71499) (Arnold et al, 2013) by replacing sgGFP with the Gal4-DNA-binding domain (DBD) followed by a poly-glycine linker upstream of the candidate library insertion site, containing the ccdB suicide gene flanked by homology arms (used for cloning the candidates during library generation; for the library cloning strategy please see ref. (Arnold et al, 2013)), which is followed by three stop codons (one for each reading-frame).
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tAD-seq Gal4-DBD

Resource Information

• Supplemental Documents:
  • Genbank- ptAD-seq-ubi63E-Gal4-DBD
• A portion of this plasmid was derived from a plasmid made by: Derived from the fly STARR-seq plasmid (AddgeneID 71499) (Arnold et al, 2013)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control result and the depositor's Genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.