Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3
(Plasmid #111925)

• Purpose: Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ
• Depositing Lab: Takeharu Nagai
• Publication: Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CeNL(Ca2+)_110μ
• Alt name: Cyan enhanced Nano-lantern (eNL (Ca2+)
• Species: Synthetic
• Insert Size (bp): 1776
• Mutation: E31D, F92W, E104D. D133E at CaM
• GenBank ID: LC334053
• Promoter: CMV
• Tags / Fusion Proteins:
  • Calreticulin (N terminal on insert)
  • KDEL (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: TTGGATCCGATGGTGAGCAAGGGCGAGGAG
• 3′ sequencing primer: atGGTACCGCCTGATCCACCCGCCAGAATGCGTTCGCACAG

Resource Information

• Supplemental Documents:
  • Gene sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry