CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3
(Plasmid #111924)

• Purpose: Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ
• Depositing Lab: Takeharu Nagai
• Publication: Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OeNL(Ca2+)_18μ
• Species: Synthetic
• Insert Size (bp): 1707
• Mutation: E67D, E104D, D133E at CaM
• GenBank ID: OeNL(Ca2+)_18μ
• Promoter: CMV
• Tag / Fusion Protein:
  • CoxVIIIx2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TTGGATCCGATGGTGAGCAAGGGCGAGGAG
• 3′ sequencing primer: ATGCTCGAGTTACGCCAGAATGCGTTCGCACAG

Resource Information

• Supplemental Documents:
  • Insert Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry