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CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3
(Plasmid #111923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CeNL(Ca2+)_19μ
  • Alt name
    Cyan enhanced Nano-lantern (eNL (Ca2+)
  • Species
    Synthetic
  • Insert Size (bp)
    1779
  • Mutation
    E67D, F92W, E104D, D133E at CaM
  • GenBank ID
    LC334052
  • Promoter CMV
  • Tag / Fusion Protein
    • CoxVIIIx2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TTGGATCCGATGGTGAGCAAGGGCGAGGAG
  • 3′ sequencing primer ATGCTCGAGTTACGCCAGAATGCGTTCGCACAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 111923 ; http://n2t.net/addgene:111923 ; RRID:Addgene_111923)

  • For your References section:

    Bioluminescent Low-Affinity Ca(2+) Indicator for ER with Multicolor Calcium Imaging in Single Living Cells. Hossain MN, Suzuki K, Iwano M, Matsuda T, Nagai T. ACS Chem Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014. 10.1021/acschembio.7b01014 PubMed 29494125
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