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pCRII-TOPO-ZFERV
(Plasmid #11192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3950
  • Vector type
    Zebrafish
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZFERV
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    11249
  • GenBank ID
    AF503912
  • Entrez Gene
    erv (a.k.a. ZFERV, si:dkey-266f7.7, wu:fd52b06, wu:fi23c07, zmp:0000000535)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO TA cloned (not destroyed)
  • 3′ cloning site TOPO TA cloned (not destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ZFERV PCR insert (the single 3' A overhangs on both ends are not included): 5'-TCTAAAGGAAAATGAACTTAACAGTTGCGAG (31 nt total upstream of the ZFERV sequence), CGAGgacaac agtggaatgg aatctttgtt aaatttagca
aagtacagta aactgaacag agtattgcga ata -3'
(73 nt total downstream of the ZFERV sequence).
A map of pCRII-TOPO is available at Addgene's vector DB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-TOPO-ZFERV was a gift from Lisa Steiner (Addgene plasmid # 11192 ; http://n2t.net/addgene:11192 ; RRID:Addgene_11192)

  • For your References section:

    Genome structure and thymic expression of an endogenous retrovirus in zebrafish. Shen CH, Steiner LA. J Virol. 2004 Jan . 78(2):899-911. 10.1128/JVI.78.2.899-911.2004 PubMed 14694121
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