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Addgene

pCB32
(Plasmid #111911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSMulti425 Hyg-Leu
  • Backbone manufacturer
    Stanford Genome Technology Center
  • Modifications to backbone
    Removed Leu marker and replaced with gRNA cassette
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA cassette targeting Nte1 locus
  • gRNA/shRNA sequence
    GTTAGTGCAAGCTTTCAAAC
  • Species
    S. cerevisiae (budding yeast)
  • Promoter SNR52p

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer agcttgtctgtaagcgga
  • 3′ sequencing primer cctatggaaaaacgccagAAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB32 was a gift from Yi Tang (Addgene plasmid # 111911 ; http://n2t.net/addgene:111911 ; RRID:Addgene_111911)
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