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ppyCAG_RNaseH1_WKKD
(Plasmid #111905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ppyCAG
  • Backbone size w/o insert (bp) 6500
  • Total vector size (bp) 7279
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNASEH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    777
  • Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to A, D274 to N
  • GenBank ID
    NM_002936
  • Entrez Gene
    RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to high GC content of the CAG promoter, there are some discrepancies in the promoter sequence. These are not known to affect the plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ppyCAG_RNaseH1_WKKD was a gift from Xiang-Dong Fu (Addgene plasmid # 111905 ; http://n2t.net/addgene:111905 ; RRID:Addgene_111905)

  • For your References section:

    R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters. Chen L, Chen JY, Zhang X, Gu Y, Xiao R, Shao C, Tang P, Qian H, Luo D, Li H, Zhou Y, Zhang DE, Fu XD. Mol Cell. 2017 Nov 16;68(4):745-757.e5. doi: 10.1016/j.molcel.2017.10.008. Epub 2017 Nov 2. 10.1016/j.molcel.2017.10.008 PubMed 29104020
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