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Addgene

pGPDluxAB(Ura)
(Plasmid #1119)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p426
  • Backbone size w/o insert (bp) 6412
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bacterial luciferase
  • Alt name
    n/a
  • Species
    bacteria
  • Insert Size (bp)
    2403
  • Mutation
    n/a
  • GenBank ID
    E12410

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer GPD
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A. Escher

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGPDluxAB(Ura) was a gift from Susan Lindquist (Addgene plasmid # 1119 ; http://n2t.net/addgene:1119 ; RRID:Addgene_1119)

  • For your References section:

    Multiple effects of trehalose on protein folding in vitro and in vivo. Singer MA, Lindquist S. Mol Cell 1998 Apr;1(5):639-48. 10.1016/S1097-2765(00)80064-7 PubMed 9660948
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