pEMS2272
(Plasmid #111898)

• Purpose: ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE
• Depositing Lab: Elizabeth Simpson
• Publication: Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.

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Backbone

• Vector backbone: pEMS2131
• Backbone manufacturer: Elizabeth Simpson (Addgene plasmid # 111895)
• Backbone size w/o insert (bp): 4999
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ple342
• Species: H. sapiens (human)
• Insert Size (bp): 1992
• Entrez Gene: TUBB3 (a.k.a. CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4)
• Promoter: Ple342 (TUBB3 MiniPromoter)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13-rev
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pEMS2272.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

. Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.