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pNAS1b split mCherry NEDD4-2 WW2
(Plasmid #111883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNAS1b
  • Backbone size (bp) 5500
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • Split mCherry system: NEDD4-2 WW2 fused to C-terminal split mCherry; phosphosite empty cassette fused to N-term split mCherry (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNAS1b split mCherry NEDD4-2 WW2 was a gift from Jesse Rinehart (Addgene plasmid # 111883 ; http://n2t.net/addgene:111883 ; RRID:Addgene_111883)

  • For your References section:

    Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J. Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11. 10.1038/nbt.4150 PubMed 29889213
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