Hook3(1-522)-GFP-Psc-StrepII
(Plasmid #111861)

• Purpose: C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.
• Depositing Lab: Andrew Carter
• Publication: Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.

Backbone

• Vector backbone: pACEBac1
• Backbone size w/o insert (bp): 2857
• Total vector size (bp): 5215
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hook3(1-552)-GFP
• Species: H. sapiens (human)
• Insert Size (bp): 2358
• Mutation: Optimised for expression in SF9 cells
• Entrez Gene: HOOK3 (a.k.a. HK3)
• Tags / Fusion Proteins:
  • Strep tag (C terminal on insert)
  • GFP (C terminal on insert)
  • Psc tag (C terminal on insert)

Resource Information

• Supplemental Documents:
  • P2063_Hook3_1_1566_GFP_Psc_StrepII.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.