Hook3(1-522)-SNAPf-Psc-StrepII
(Plasmid
#111860)
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PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACEBac1
- Backbone size w/o insert (bp) 2857
- Total vector size (bp) 5071
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHook3(1-552)-SNAPf
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SpeciesH. sapiens (human)
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Insert Size (bp)2214
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MutationOptimised for expression in Sf9 cells
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Entrez GeneHOOK3 (a.k.a. HK3)
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Tags
/ Fusion Proteins
- SNAPf (C terminal on insert)
- Psc (C terminal on insert)
- Strep-II (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hook3(1-522)-SNAPf-Psc-StrepII was a gift from Andrew Carter (Addgene plasmid # 111860 ; http://n2t.net/addgene:111860 ; RRID:Addgene_111860) -
For your References section:
Cryo-EM shows how dynactin recruits two dyneins for faster movement. Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP. Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462. 10.1038/nature25462 PubMed 29420470
Map uploaded by the depositor.
