His-ZZ-TEV-SNAPf-BICD2N(1-400)
(Plasmid #111857)

• Purpose: N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.
• Depositing Lab: Andrew Carter
• Publication: Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462.

Backbone

• Vector backbone: pOmnibac
• Backbone size w/o insert (bp): 4517
• Total vector size (bp): 6813
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BICD2N(1-400)
• Species: M. musculus (mouse)
• Insert Size (bp): 2296
• Mutation: Optimised for Sf9 expression
• Tags / Fusion Proteins:
  • ZZ (N terminal on insert)
  • TEV site (N terminal on insert)
  • His-tag (N terminal on insert)
  • SNAPf-tag (N terminal on insert)

Resource Information

• Supplemental Documents:
  • p1057 ZZ-LTL-SNAPf-BICD2Nopt.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.