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Addgene

pVEG17*
(Plasmid #111856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415
  • Backbone size w/o insert (bp) 5970
  • Total vector size (bp) 6021
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL1p-GAL3-Syn4.1-TEFt
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2482
  • Mutation
    D68N, V69M, A109V, I271L
  • Promoter GAL1p

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gtgctgcaaggcgattaag
  • 3′ sequencing primer cactttatgcttccggctc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVEG17* was a gift from Nikhil Nair (Addgene plasmid # 111856 ; http://n2t.net/addgene:111856 ; RRID:Addgene_111856)

  • For your References section:

    A semi-synthetic regulon enables rapid growth of yeast on xylose. Endalur Gopinarayanan V, Nair NU. Nat Commun. 2018 Mar 26;9(1):1233. doi: 10.1038/s41467-018-03645-7. 10.1038/s41467-018-03645-7 [pii] PubMed 29581426
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