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Addgene

pDNA2.0 6H-TEV-KRAS G13D
(Plasmid #111851)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111851 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDNA2.0
  • Backbone manufacturer
    ATUM
  • Backbone size w/o insert (bp) 3939
  • Total vector size (bp) 4500
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KRAS G13D
  • Alt name
    Kirsten ras oncogene G13D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    507
  • Mutation
    Changed Glycine 13 to Aspartic Acid
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter T5
  • Tags / Fusion Proteins
    • 6xHis-tag (N terminal on backbone)
    • TEV protease cleavage sequence (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGAATTGTGAGCGCTCACAA
  • 3′ sequencing primer GAACTGCCAGGCATCAAATAAAA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDNA2.0 6H-TEV-KRAS G13D was a gift from Kenneth Westover (Addgene plasmid # 111851 ; http://n2t.net/addgene:111851 ; RRID:Addgene_111851)
  • For your References section:

    Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations. Hunter JC, Manandhar A, Carrasco MA, Gurbani D, Gondi S, Westover KD. Mol Cancer Res. 2015 Sep;13(9):1325-35. doi: 10.1158/1541-7786.MCR-15-0203. Epub 2015 Jun 2. 10.1158/1541-7786.MCR-15-0203 PubMed 26037647