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pCEP4-BG505.SOSIP-QES.i03.c01-8his
(Plasmid #111846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10393
  • Total vector size (bp) 12427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 Env (BG505 SOSIP.664)
  • Alt name
    gp140
  • Species
    Human immunodeficiency virus 1
  • Insert Size (bp)
    2034
  • Mutation
    Codon-optimized synthetic gene; has SOSIP mutations T332N;A501C;I559P;T605C;6xArg cleavage site; has QES mutations V181L;A200E;F223Y;V255M;I595M
  • GenBank ID
    ABA61516.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • CD5 leader sequence (N terminal on insert)
    • 8his purification tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-BG505.SOSIP-QES.i03.c01-8his was a gift from Erik Procko (Addgene plasmid # 111846 ; http://n2t.net/addgene:111846 ; RRID:Addgene_111846)

  • For your References section:

    Conformational engineering of HIV-1 Env based on mutational tolerance in the CD4 and PG16 bound states. Heredia JD, Park J, Choi H, Gill KS, Procko E. J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19. 10.1128/JVI.00219-19 PubMed 30894475
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