pCEP4-BG505.SOSIP-QES.i03.c01-TM
(Plasmid #111845)

• Purpose: Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16
• Depositing Lab: Erik Procko
• Publication: Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10393
• Total vector size (bp): 12541
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIV-1 Env (BG505 SOSIP.664)
• Alt name: gp140
• Species: Human immunodeficiency virus 1
• Insert Size (bp): 2148
• Mutation: Codon-optimized synthetic gene; has SOSIP mutations T332N;A501C;I559P;T605C;6xArg cleavage site; has QES mutations V181L;A200E;F223Y;V255M;I595M
• GenBank ID: ABA61516.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • CD5 leader sequence (N terminal on insert)
  • TM helix from MHC class I (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry