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PurposeFor mamalian expression of Ecdysone receptor-based inducible transgene expression switch.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111834 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPur
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Backbone manufacturerClonetech
- Total vector size (bp) 8166
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGAL4-minimal VP16-EcR
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SpeciesSynthetic
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Entrez GeneEcR (a.k.a. Dmel_CG1765, CG1765, CG8347, DEcR, Dhr23, DmEcR, Dmel\CG1765, ECR, ECR-C, EcR-A, EcR-B, EcR-B1, EcR1b, EcRA, EcRB, EcRB-1, EcRB1, EcRC, EcdR, Ecr, Ecr1B, EcrB1, NR1H1, USP, Usp, anon-WO0229075.1, dECR, dmEcR, ecr, lie, ms(2)06410, ms(2)42A, snt)
- Promoter CMV, SV40, 10XUAS
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEUI(+) was a gift from Hyunju Ro (Addgene plasmid # 111834 ; http://n2t.net/addgene:111834 ; RRID:Addgene_111834) -
For your References section:
Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues. Lee S, Sohn KC, Choi DK, Won M, Park KA, Ju SK, Kang K, Bae YK, Hur GM, Ro H. Mol Ther Nucleic Acids. 2016 Sep 27;5(9):e367. doi: 10.1038/mtna.2016.74. 10.1038/mtna.2016.74 PubMed 27673563