pRRL-VinculinTS-I997A
(Plasmid #111832)

• Purpose: Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector
• Depositing Lab: Brenton Hoffman
• Publication: Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 10.1016/j.bpj.2018.02.019.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 6235
• Total vector size (bp): 11602
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VinculinTS (I997A)
• Species: G. gallus (chicken)
• Insert Size (bp): 5367
• Mutation: changed vinculin isoleucine 997 to alanine
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GGTACAGTGCAGGGGAAAG
• 3′ sequencing primer: GTTAAGAATACCAGTCAATCTTTCAC

Resource Information

• Supplemental Documents:
  • prrl-vints-i997a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry