pFLAG rat Antizyme 1
(Plasmid
#11183)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAZ1
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Alt nameornithine decarboxylase antizyme 1
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Alt nameantizyme 1
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Alt nameOAZ1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)700
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GenBank IDNM_139081
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Entrez GeneOaz1 (a.k.a. ODC-Az, ODCAC, Oaz)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG rat Antizyme 1 was a gift from Bruce Zetter (Addgene plasmid # 11183 ; http://n2t.net/addgene:11183 ; RRID:Addgene_11183) -
For your References section:
Antizyme targets cyclin D1 for degradation. A novel mechanism for cell growth repression. Newman RM, Mobascher A, Mangold U, Koike C, Diah S, Schmidt M, Finley D, Zetter BR. J Biol Chem. 2004 Oct 1. 279(40):41504-11. 10.1074/jbc.M407349200 PubMed 15277517
Map uploaded by the depositor.
