VinculinTS-A50I/I997A
(Plasmid #111829)

• Purpose: Vinculin tension sensor with point mutations affecting ability to bind talin and actin
• Depositing Lab: Brenton Hoffman
• Publication: Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 10.1016/j.bpj.2018.02.019.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5363
• Total vector size (bp): 10118
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VinculinTS (I997A)
• Species: G. gallus (chicken)
• Insert Size (bp): 4755
• Mutation: changed vinculin alanine 50 to isoleucine and isoleucine 997 to alanine
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pcdna31-vints-a50i-i997a.gb
• A portion of this plasmid was derived from a plasmid made by: Susan Craig (Addgene #46269) & Sharon Campbell (Template DNA: pET15b GgVcl 884-1066 ΔAB)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry