Skip to main content
Addgene

VinculinTS-A50I
(Plasmid #111827)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111827 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5402
  • Total vector size (bp) 10157
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VinculinTS (A50I)
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    4755
  • Mutation
    changed alanine 50 to isoleucine
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Susan Craig (Addgene #46269)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VinculinTS-A50I was a gift from Brenton Hoffman (Addgene plasmid # 111827 ; http://n2t.net/addgene:111827 ; RRID:Addgene_111827)
  • For your References section:

    Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration. Rothenberg KE, Scott DW, Christoforou N, Hoffman BD. Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 10.1016/j.bpj.2018.02.019. 10.1016/j.bpj.2018.02.019 PubMed 29642037