VinculinTS-A50I
(Plasmid
#111827)
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PurposeVinculin tension sensor with point mutation affecting ability to bind talin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5402
- Total vector size (bp) 10157
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVinculinTS (A50I)
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SpeciesG. gallus (chicken)
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Insert Size (bp)4755
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Mutationchanged alanine 50 to isoleucine
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySusan Craig (Addgene #46269)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VinculinTS-A50I was a gift from Brenton Hoffman (Addgene plasmid # 111827 ; http://n2t.net/addgene:111827 ; RRID:Addgene_111827) -
For your References section:
Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration. Rothenberg KE, Scott DW, Christoforou N, Hoffman BD. Biophys J. 2018 Apr 10;114(7):1680-1694. doi: 10.1016/j.bpj.2018.02.019. 10.1016/j.bpj.2018.02.019 PubMed 29642037