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Addgene

pT7sgRNA
(Plasmid #111820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgRNA
  • Species
    Streptococcus pyogenes

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7sgRNA was a gift from David Horn (Addgene plasmid # 111820 ; http://n2t.net/addgene:111820 ; RRID:Addgene_111820)

  • For your References section:

    Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes. Rico E, Jeacock L, Kovarova J, Horn D. Sci Rep. 2018 May 21;8(1):7960. doi: 10.1038/s41598-018-26303-w. 10.1038/s41598-018-26303-w PubMed 29785042
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