pcDNA cyclin D1 HA T286A
(Plasmid #11182)

• Depositing Lab: Bruce Zetter
• Publication: Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-11.

Backbone

• Vector backbone: pcDNA3?
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D1 T286A
• Alt name: cyclin D1
• Species: H. sapiens (human)
• Insert Size (bp): 1120
• Mutation: T286A. Mutant that cannot be degraded via the ubiquitin pathway.
• GenBank ID: BC025302
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed using site-directed mutagenesis on the parent plasmid, pcDNA cyclinD1 HA, using the primers 5'-acctggcttgcgcacccaccgacg-3' and 5'-cgtcggtgggtgcgcaagccaggt-3'.

Note: The author's map and author's sequence are not fully correct. EcoRI and XhoI are not unique sites in this plasmid.