pRPaCas9
(Plasmid
#111819)
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PurposeExpresses Cas9 in T. brucei (2T1) cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9
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SpeciesStreptococcus pyogenes
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GenBank IDQ99ZW2.1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRPaCas9 was a gift from David Horn (Addgene plasmid # 111819 ; http://n2t.net/addgene:111819 ; RRID:Addgene_111819) -
For your References section:
Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes. Rico E, Jeacock L, Kovarova J, Horn D. Sci Rep. 2018 May 21;8(1):7960. doi: 10.1038/s41598-018-26303-w. 10.1038/s41598-018-26303-w PubMed 29785042
Map uploaded by the depositor.
