pMXs-PT2A-iRFP670-GFP-Tdtomato
(Plasmid #111816)

• Purpose: Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A
• Depositing Lab: Li Qian
• Publication: Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2.

Backbone

• Vector backbone: pMXs
• Backbone manufacturer: Cellbiolabs
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 7800
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iRFP670
• Insert Size (bp): 900

Gene/Insert 2

• Gene/Insert name: GFP
• Insert Size (bp): 700

Gene/Insert 3

• Gene/Insert name: Tdtomato
• Insert Size (bp): 1400

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

NheI right after iRFP670 was destroyed during cloning, containing half SpeI and half NheI.