pMXs-PT2A-GFP-iRFP670-Tdtomato
(Plasmid
#111813)
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PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
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Backbone manufacturerCellbiolabs
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 7800
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)HB101
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameGFP
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Insert Size (bp)700
Gene/Insert 2
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Gene/Insert nameiRFP670
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Insert Size (bp)900
Gene/Insert 3
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Gene/Insert nameTdtomato
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Insert Size (bp)1400
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-PT2A-GFP-iRFP670-Tdtomato was a gift from Li Qian (Addgene plasmid # 111813 ; http://n2t.net/addgene:111813 ; RRID:Addgene_111813) -
For your References section:
Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819