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Addgene

pMXs-PT2A-GFP-iRFP670-Tdtomato
(Plasmid #111813)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111813 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMXs
  • Backbone manufacturer
    Cellbiolabs
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HB101
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    700

Gene/Insert 2

  • Gene/Insert name
    iRFP670
  • Insert Size (bp)
    900

Gene/Insert 3

  • Gene/Insert name
    Tdtomato
  • Insert Size (bp)
    1400

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-PT2A-GFP-iRFP670-Tdtomato was a gift from Li Qian (Addgene plasmid # 111813 ; http://n2t.net/addgene:111813 ; RRID:Addgene_111813)
  • For your References section:

    Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819