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pUAST-attB-Flag-dTSC2 WT
(Plasmid #111807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-attB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dTSC2
  • Alt name
    gig
  • Alt name
    gigas
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    gig (a.k.a. Dmel_CG6975, 6975, C1, CG6975, Dmel\CG6975, FBpp0074588, Gigas, ME 109, TSC2, TsC2, Tsc2, Tsc2/gig, dTSC2, dTsc2, gig/Tsc2, l(3)109, tsc2, tsc2(gig))
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer 5' pUAST
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-attB-Flag-dTSC2 WT was a gift from Jun Hee Lee (Addgene plasmid # 111807 ; http://n2t.net/addgene:111807 ; RRID:Addgene_111807)

  • For your References section:

    Identification of an AMPK phosphorylation site in Drosophila TSC2 (gigas) that regulate cell growth. Kim M, Lee JH. Int J Mol Sci. 2015 Mar 27;16(4):7015-26. doi: 10.3390/ijms16047015. 10.3390/ijms16047015 PubMed 25826530
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