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Addgene

pUAST-Myc-dATG1
(Plasmid #111801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dAtg1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Atg1 (a.k.a. Dmel_CG10967, ATG1, CG10967, DK-4, DmATG1, Dmel\CG10967, ULK1, UNC 51-like, Unc-51, Unc51, anon-WO0118547.287, atg, atg-1, atg1, dATG1, dAtg1, l(3)00305, unc-51, unc51)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer 5' pUAST
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-Myc-dATG1 was a gift from Jun Hee Lee (Addgene plasmid # 111801 ; http://n2t.net/addgene:111801 ; RRID:Addgene_111801)

  • For your References section:

    Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging. Kim M, Park HL, Park HW, Ro SH, Nam SG, Reed JM, Guan JL, Lee JH. Autophagy. 2013 Aug;9(8):1201-13. doi: 10.4161/auto.24811. Epub 2013 May 14. 10.4161/auto.24811 PubMed 23819996
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