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Addgene

pcDNA-Flag-Sesn-A mSesn2 (delBC)
(Plasmid #111797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mSesn2
  • Species
    M. musculus (mouse)
  • Mutation
    BC domain truncation
  • Entrez Gene
    Sesn2 (a.k.a. HI95, SEST2, Ses2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Flag-Sesn-A mSesn2 (delBC) was a gift from Jun Hee Lee (Addgene plasmid # 111797 ; http://n2t.net/addgene:111797 ; RRID:Addgene_111797)

  • For your References section:

    Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1. Ro SH, Semple IA, Park H, Park H, Park HW, Kim M, Kim JS, Lee JH. FEBS J. 2014 Sep;281(17):3816-27. doi: 10.1111/febs.12905. Epub 2014 Jul 28. 10.1111/febs.12905 PubMed 25040165
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