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Addgene

pLU-CMV-Flag-hSesn2 R404A/D406A/D407A
(Plasmid #111790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLU-CMV
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hSesn2
  • Species
    H. sapiens (human)
  • Mutation
    R404A/D406A/D407A
  • Entrez Gene
    SESN2 (a.k.a. HI95, SES2, SEST2)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLU-CMV-Flag-hSesn2 R404A/D406A/D407A was a gift from Jun Hee Lee (Addgene plasmid # 111790 ; http://n2t.net/addgene:111790 ; RRID:Addgene_111790)

  • For your References section:

    Janus-faced Sestrin2 controls ROS and mTOR signalling through two separate functional domains. Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, Jakob U, Lee JH, Cho US. Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025. 10.1038/ncomms10025 PubMed 26612684
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