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Purpose(Empty Backbone) Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEMT-easy
- Backbone size (bp) 3200
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Modifications to backboneReplaced the original MCS with 3P2A using AatII and BstXI
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Vector typecloning intermediate
- Promoter T7
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 F/T7
- 3′ sequencing primer M13 R/SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEMT-PTE2A was a gift from Li Qian (Addgene plasmid # 111773 ; http://n2t.net/addgene:111773 ; RRID:Addgene_111773) -
For your References section:
Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819
Map uploaded by the depositor.
