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Addgene

pGEMT-PTE2A
(Plasmid #111773)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111773 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEMT-easy
  • Backbone size (bp) 3200
  • Modifications to backbone
    Replaced the original MCS with 3P2A using AatII and BstXI
  • Vector type
    cloning intermediate
  • Promoter T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-PTE2A was a gift from Li Qian (Addgene plasmid # 111773 ; http://n2t.net/addgene:111773 ; RRID:Addgene_111773)
  • For your References section:

    Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819