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Addgene

pcDNA3/BLM
(Plasmid #111766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen (thermoFisher)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BLM
  • Alt name
    BS
  • Alt name
    RECQ2
  • Species
    H. sapiens (human)
  • Entrez Gene
    BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/BLM was a gift from Ian Hickson (Addgene plasmid # 111766 ; http://n2t.net/addgene:111766 ; RRID:Addgene_111766)

  • For your References section:

    Increased error-prone non homologous DNA end-joining--a proposed mechanism of chromosomal instability in Bloom's syndrome. Gaymes TJ, North PS, Brady N, Hickson ID, Mufti GJ, Rassool FV. Oncogene. 2002 Apr 11;21(16):2525-33. doi: 10.1038/sj.onc.1205331. 10.1038/sj.onc.1205331 PubMed 11971187
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