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Addgene

VinTS-tCRMod-GGSGGS9
(Plasmid #111765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5399
  • Total vector size (bp) 10166
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
  • Alt name
    VinTS-tCRMod-GGSGGS9
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    4767
  • Mutation
    Residues 1-228 of Clover, residues 1-3 deleted from mRuby2
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Partially based on Addgene Plasmids #26019, #49089, and #21769

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VinTS-tCRMod-GGSGGS9 was a gift from Brenton Hoffman (Addgene plasmid # 111765 ; http://n2t.net/addgene:111765 ; RRID:Addgene_111765)

  • For your References section:

    Tunable molecular tension sensors reveal extension-based control of vinculin loading. LaCroix AS, Lynch AD, Berginski ME, Hoffman BD. eLife. 2018 Jul 19;7. pii: 33927. doi: 10.7554/eLife.33927. 10.7554/eLife.33927 PubMed 30024378
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