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Addgene

DOT1L-CC3
(Plasmid #111754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-MKH8SUMO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DOT1L
  • Alt name
    DOT1 like histone lysine methyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    147
  • Mutation
    Contains coiled coil #3 (residues 613-661)
  • Entrez Gene
    DOT1L (a.k.a. DOT1, KMT4)
  • Promoter T7
  • Tag / Fusion Protein
    • 8xHis-Thrombin-SUMO-TEV (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic gene ordered from GeneScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DOT1L-CC3 was a gift from Cheryl Arrowsmith (Addgene plasmid # 111754 ; http://n2t.net/addgene:111754 ; RRID:Addgene_111754)

  • For your References section:

    Structural and functional analysis of the DOT1L-AF10 complex reveals mechanistic insights into MLL-AF10-associated leukemogenesis. Zhang H, Zhou B, Qin S, Xu J, Harding R, Tempel W, Nayak V, Li Y, Loppnau P, Dou Y, Min J. Genes Dev. 2018 Mar 1;32(5-6):341-346. doi: 10.1101/gad.311639.118. Epub 2018 Mar 21. 10.1101/gad.311639.118 PubMed 29563185
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