DOT1L-CC3
(Plasmid
#111754)
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PurposeBacterial expression for structure determination
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MKH8SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDOT1L
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Alt nameDOT1 like histone lysine methyltransferase
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SpeciesH. sapiens (human)
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Insert Size (bp)147
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MutationContains coiled coil #3 (residues 613-661)
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Entrez GeneDOT1L (a.k.a. DOT1, KMT4)
- Promoter T7
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Tag
/ Fusion Protein
- 8xHis-Thrombin-SUMO-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysynthetic gene ordered from GeneScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DOT1L-CC3 was a gift from Cheryl Arrowsmith (Addgene plasmid # 111754 ; http://n2t.net/addgene:111754 ; RRID:Addgene_111754) -
For your References section:
Structural and functional analysis of the DOT1L-AF10 complex reveals mechanistic insights into MLL-AF10-associated leukemogenesis. Zhang H, Zhou B, Qin S, Xu J, Harding R, Tempel W, Nayak V, Li Y, Loppnau P, Dou Y, Min J. Genes Dev. 2018 Mar 1;32(5-6):341-346. doi: 10.1101/gad.311639.118. Epub 2018 Mar 21. 10.1101/gad.311639.118 PubMed 29563185