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Addgene

AF10_OM-LZ
(Plasmid #111753)

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Item Catalog # Description Quantity Price (USD)
Plasmid 111753 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    Arrowsmith lab (Addgene plasmid# 26096)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MLLT10
  • Alt name
    PDB ID: 6CKN
  • Alt name
    gi|57546900
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    228
  • Mutation
    Contains the OM-LZ region of AF10 (residues 720-795)
  • Entrez Gene
    MLLT10 (a.k.a. AF10)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His - TEV (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N-Tag:MHHHHHHSSGRENLYFQG. MLLT10:JMC076-B05:C227962.
SGC link: http://www.thesgc.org/structures/6CKN/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AF10_OM-LZ was a gift from Cheryl Arrowsmith (Addgene plasmid # 111753 ; http://n2t.net/addgene:111753 ; RRID:Addgene_111753)

  • For your References section:

    Structural and functional analysis of the DOT1L-AF10 complex reveals mechanistic insights into MLL-AF10-associated leukemogenesis. Zhang H, Zhou B, Qin S, Xu J, Harding R, Tempel W, Nayak V, Li Y, Loppnau P, Dou Y, Min J. Genes Dev. 2018 Mar 1;32(5-6):341-346. doi: 10.1101/gad.311639.118. Epub 2018 Mar 21. 10.1101/gad.311639.118 PubMed 29563185
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