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Addgene

137 pGEX2TK RBAP2 HA
(Plasmid #11174)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Entrez Gene
    ARID4A (a.k.a. RBBP-1, RBBP1, RBP-1, RBP1)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX-5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    137 pGEX2TK RBAP2 HA was a gift from William Sellers (Addgene plasmid # 11174 ; http://n2t.net/addgene:11174 ; RRID:Addgene_11174)
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