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pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q142
(Plasmid #111739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111739 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBacMam2-DiEx-LIC
  • Vector type
    Mammalian Expression ; Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTT
  • Alt name
    huntingtin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9789
  • Mutation
    Q142 (polyQ repeat)
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Promoter CMV and P10
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBM-fwd (5'-caaaatgtcgtaacaactccgc-3')
  • 3′ sequencing primer pFBM-rev (5'- tagttaagaataccagtcaatctttcac-3')
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kazusa DNA Research Institute Gene KIEE4296 & Original Clone ID cp94296

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q142 was a gift from Cheryl Arrowsmith (Addgene plasmid # 111739)

  • For your References section:

    Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH. J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 10.1074/jbc.RA118.007204. Epub 2019 Mar 6. 10.1074/jbc.RA118.007204 PubMed 30842263
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