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Addgene

pJK1
(Plasmid #111722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BLM
  • Alt name
    BS
  • Alt name
    RECQ2
  • Species
    H. sapiens (human)
  • Mutation
    a C-terminal hexahistidine tag was added, a yeast Kozak consensus sequence was included upstream of the coding sequence, and the first five codons of S. cerevisiae TOP2 gene replaced the initiation codon of the BLM cDNA.
  • Entrez Gene
    BLM (a.k.a. BS, MGRISCE1, RECQ2, RECQL2, RECQL3)
  • Promoter GAL1
  • Tag / Fusion Protein
    • hexahistidine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK1 was a gift from Ian Hickson (Addgene plasmid # 111722 ; http://n2t.net/addgene:111722 ; RRID:Addgene_111722)

  • For your References section:

    The Bloom's syndrome gene product is a 3'-5' DNA helicase. Karow JK, Chakraverty RK, Hickson ID. J Biol Chem. 1997 Dec 5;272(49):30611-4. PubMed 9388193
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