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Designed HEL-binding scFv D44.1des
(Plasmid #111719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCTcon2
  • Backbone size w/o insert (bp) 6168
  • Total vector size (bp) 6945
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D44.1 des
  • Species
    Synthetic
  • Insert Size (bp)
    777
  • Mutation
    L Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H N102Y, H G104K
  • Tag / Fusion Protein
    • cmyc tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer GCAGCCCCATAAACACACAGTATG
  • 3′ sequencing primer GGAGAAATGAAAAGTATATTGTATTTTGTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Designed HEL-binding scFv D44.1des was a gift from Sarel Fleishman (Addgene plasmid # 111719 ; http://n2t.net/addgene:111719 ; RRID:Addgene_111719)

  • For your References section:

    Optimizing antibody affinity and stability by the automated design of the variable light-heavy chain interfaces. Warszawski S, Borenstein Katz A, Lipsh R, Khmelnitsky L, Ben Nissan G, Javitt G, Dym O, Unger T, Knop O, Albeck S, Diskin R, Fass D, Sharon M, Fleishman SJ. PLoS Comput Biol. 2019 Aug 23;15(8):e1007207. doi: 10.1371/journal.pcbi.1007207. eCollection 2019 Aug. 10.1371/journal.pcbi.1007207 PubMed 31442220
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