MAC_C_PDK1
(Plasmid
#111672)
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PurposeMAC-tagged gene expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMAC-tag-C
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Backbone manufacturerMarkku Varjosalo, Addgene plasmid #108077
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDK1
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Alt namePDK1
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SpeciesH. sapiens (human)
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Entrez GenePDK1
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CCSB-DFCI hORFeome collection(11062_G08)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC_C_PDK1 was a gift from Markku Varjosalo (Addgene plasmid # 111672 ; http://n2t.net/addgene:111672 ; RRID:Addgene_111672) -
For your References section:
An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, Varjosalo M. Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2. 10.1038/s41467-018-03523-2 PubMed 29568061