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Addgene

MAC_C_PDK1
(Plasmid #111672)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111672 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MAC-tag-C
  • Backbone manufacturer
    Markku Varjosalo, Addgene plasmid #108077
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDK1
  • Alt name
    PDK1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PDK1

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CCSB-DFCI hORFeome collection(11062_G08)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC_C_PDK1 was a gift from Markku Varjosalo (Addgene plasmid # 111672 ; http://n2t.net/addgene:111672 ; RRID:Addgene_111672)
  • For your References section:

    An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, Varjosalo M. Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2. 10.1038/s41467-018-03523-2 PubMed 29568061