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pMIH-TOMM20-Halo
(Plasmid #111626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIH/MSCV-IRES-hygro
  • Backbone manufacturer
    Mark Van Delft
  • Backbone size w/o insert (bp) 6969
  • Total vector size (bp) 8340
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TOMM20
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2782
  • GenBank ID
    NM_014765.2
  • Entrez Gene
    Tomm20 (a.k.a. 1810060K07Rik, BB284719, Gm19268, MAS20, MOM19, TOM20, mKIAA0016)
  • Promoter MSCV
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer gaacctcctcgttcgaccc
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Backbone provided by Dr Mark Van Delft & Prof David Huang. TOMM20 insert synthesised by Genscript (NM_014765.2). HaloTag purchased from Promega #G7721 pHTN.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIH-TOMM20-Halo was a gift from Benjamin Kile (Addgene plasmid # 111626 ; http://n2t.net/addgene:111626 ; RRID:Addgene_111626)

  • For your References section:

    BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot V, Geoghegan ND, Chappaz S, Davidson S, San Chin H, Lane RM, Dramicanin M, Saunders TL, Sugiana C, Lessene R, Osellame LD, Chew TL, Dewson G, Lazarou M, Ramm G, Lessene G, Ryan MT, Rogers KL, van Delft MF, Kile BT. Science. 2018 Feb 23;359(6378). pii: 359/6378/eaao6047. doi: 10.1126/science.aao6047. 10.1126/science.aao6047 PubMed 29472455
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