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Addgene

pTn7CdrA-gfpC
(Plasmid #111616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBK-miniTn7-omegaGm (pUC-based)
  • Vector type
    Bacterial Expression
  • Selectable markers
    Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PcdrA-RBSII-gfp(Mut3)-T0-T1
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7CdrA-gfpC was a gift from Tim Tolker-Nielsen (Addgene plasmid # 111616 ; http://n2t.net/addgene:111616 ; RRID:Addgene_111616)

  • For your References section:

    Fluorescence-based reporter for gauging cyclic di-GMP levels in Pseudomonas aeruginosa. Rybtke MT, Borlee BR, Murakami K, Irie Y, Hentzer M, Nielsen TE, Givskov M, Parsek MR, Tolker-Nielsen T. Appl Environ Microbiol. 2012 Aug;78(15):5060-9. doi: 10.1128/AEM.00414-12. Epub 2012 May 11. 10.1128/AEM.00414-12 PubMed 22582064
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