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Addgene

pPBAD-yhjH
(Plasmid #111613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJN105 (pBBR1-based)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    yhjH
  • Mutation
    A52V

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A52V mutation in yhjH does not alter enzyme function. The expressed YhjH enzyme is a functional phosphodiesterase that degrades cyclic di-GMP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBAD-yhjH was a gift from Tim Tolker-Nielsen (Addgene plasmid # 111613 ; http://n2t.net/addgene:111613 ; RRID:Addgene_111613)

  • For your References section:

    Clearance of Pseudomonas aeruginosa foreign-body biofilm infections through reduction of the cyclic Di-GMP level in the bacteria. Christensen LD, van Gennip M, Rybtke MT, Wu H, Chiang WC, Alhede M, Hoiby N, Nielsen TE, Givskov M, Tolker-Nielsen T. Infect Immun. 2013 Aug;81(8):2705-13. doi: 10.1128/IAI.00332-13. Epub 2013 May 20. 10.1128/IAI.00332-13 PubMed 23690403
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