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Addgene

pDAI-SceI-pheS
(Plasmid #111611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDAI-SceI (pBBR1-based)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pheS
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDAI-SceI-pheS was a gift from Tim Tolker-Nielsen (Addgene plasmid # 111611 ; http://n2t.net/addgene:111611 ; RRID:Addgene_111611)

  • For your References section:

    In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology. Fazli M, Harrison JJ, Gambino M, Givskov M, Tolker-Nielsen T. Appl Environ Microbiol. 2015 Jun;81(11):3623-30. doi: 10.1128/AEM.03909-14. Epub 2015 Mar 20. 10.1128/AEM.03909-14 PubMed 25795676
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