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Addgene

pDONRPEX18Tp-SceI-pheS
(Plasmid #111608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEX18Tp-pheS (pUC-based)
  • Vector type
    Allelic exchange in Burkholderia sp.

Growth in Bacteria

  • Bacterial Resistance(s)
    Trimethoprim
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONRPEX18Tp-SceI-pheS was a gift from Tim Tolker-Nielsen (Addgene plasmid # 111608 ; http://n2t.net/addgene:111608 ; RRID:Addgene_111608)

  • For your References section:

    In-Frame and Unmarked Gene Deletions in Burkholderia cenocepacia via an Allelic Exchange System Compatible with Gateway Technology. Fazli M, Harrison JJ, Gambino M, Givskov M, Tolker-Nielsen T. Appl Environ Microbiol. 2015 Jun;81(11):3623-30. doi: 10.1128/AEM.03909-14. Epub 2015 Mar 20. 10.1128/AEM.03909-14 PubMed 25795676
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