pCAGEN
(Plasmid #11160)

• Purpose: Mammalian expression vector for cloning and expressing your gene under the CAG promoter
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4698
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: modified multiple cloning sites
• Insert Size (bp): 100

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCAGGS (Niwa et al. Gene 108, 193-199 (1991)) was obtained from Dr. J. Miyazaki (Osaka University).
• Articles Citing this Plasmid:
  • 79 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The multiple cloning sites of pCAGGS was modified to make pCAGEN (pCAGGS with EcoRI, XhoI, EcoRV and NotI sites).

Note that the Addgene quality control sequence and the author-supplied sequence do not match exactly, but critical features (such as the MCS restriction sites) appear to be intact. If you believe there is something functionally wrong with this plasmid, please report your concern to

Information for Cloning Grade DNA (Catalog # 11160-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here