pCAGIG
(Plasmid #11159)

• Purpose: Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4817
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRES-EGFP
• Alt name: internal ribosomal entry site from Encephalomyocarditis virus
• Alt name: green fluorescent protein from Aequorea victoria
• Insert Size (bp): 1318

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: MscI (destroyed during cloning)
• 5′ sequencing primer: pCAG-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: IRES-GFP was from pMX-IRES-GFP (Nosaka et al., EMBO J. 18, 4754-4765 (1999)) obtained from Dr. T. Kitamura (Univ. of Tokyo).
• Articles Citing this Plasmid:
  • 76 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PstI site at 3112 and the HindIII site at 3120 shown in the author's map and sequence are not present in this plasmid.

Information for Cloning Grade DNA (Catalog # 11159-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here