pAAV-TIWB-yellow pre-eGRASP(p30)
(Plasmid #111588)

• Purpose: An AAV vector that expresses yellow pre-eGRASP(p30) under the tetO promoter.
• Depositing Lab: Bong-Kiun Kaang
• Publication: Choi et al Science. 2018 Apr 27;360(6387):430-435. doi: 10.1126/science.aas9204.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4375
• Total vector size (bp): 5725
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: yellow pre-eGRASP(p30)
• Insert Size (bp): 1350
• Promoter: tetO

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • pAAV-TIWB-kSPOPTySp30Nrx.gb
  • TRE3G plasmid schematic
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry