pCralbp-DsRed
(Plasmid #11158)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 3086
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cralbp promoter
• Alt name: Cellular retinaldehyde binding protein promoter
• Alt name: Rlbp1 promoter
• Alt name: Rlbp1
• Species: M. musculus (mouse)
• Insert Size (bp): 4002
• Entrez Gene: Rlbp1 (a.k.a. 3110056M11Rik, CRALBP)
• Tag / Fusion Protein:
  • DsRed2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: DsRed-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Cralbp promoter is specific for Muller glial cells in the retina